We narrowed to 23,671 results for: crispr

1. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CMV-NLS-dCas9-NLS

   Plasmid

   #99908

   Purpose

   For the expression of NLS-dCas9-NLS

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-PGK- dCas9-SunTag-P2A-HygR

   Plasmid

   #193137

   Purpose

   Expresses dCas9 fused to the SunTag scaffold

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   dCas9-SunTag-T2A-HygR
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh123

   Plasmid

   #106790

   Purpose

   expression of gRNA targeting ST8SIA3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ST8SIA3 (ST8SIA3 Human)
   

   Expression

   Mammalian

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB025

   Plasmid

   #183092

   Purpose

   Expresses FnCas12a in Bacteroides and used for genome editing

   Depositor

   Lei Dai

   Article

   Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

   Insert

   FnCas12a, gRNA, Promoter, HAB, TetR,
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1TDPGH023

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCpf1-2NLS

    Plasmid

    #102566

    Purpose

    bacterial expression of LbCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    LbCpf1
    

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV270

    Plasmid

    #226181

    Purpose

    Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p (Komagataella phaffii)

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Expression

    Worm

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

    Plasmid

    #132406

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCH67 (crRNA expression vector)

    Plasmid

    #217337

    Purpose

    CROP-seq crRNA expression vector with GFP and puro marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-puro
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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