We narrowed to 15,730 results for: NOL

1. pv9_CBX1_2xChromo-13XL-BASU

   Plasmid

   #179412

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1.2xChromo-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-Gluc-RMA-IRES-EGFP

   Plasmid

   #189629

   Purpose

   Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Promoter

   IRES and hSyn

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

   Plasmid

   #140534

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G) mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-YE1-BE4max

   Plasmid

   #198546

   Purpose

   Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY1173_ADAR1p150

   Plasmid

   #193192

   Purpose

   Express full length ADAR1p150

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   ADAR1p150 (ADAR Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/er-(n2)oxStayGold(c4)v2.0

   Plasmid

   #186296

   Purpose

   A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)v2.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-NG-ABE8e(V106W)

   Plasmid

   #198533

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

   Plasmid

   #160736

   Purpose

   Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. A2_uSFFV

    Plasmid

    #153415

    Purpose

    Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    A*02:01-P2A-human beta2 microglobulin (B2M Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-10xUAS-Voltron

    Plasmid

    #119039

    Purpose

    UAS-driven expresion of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Promoter

    E1b

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Voltron-ST

    Plasmid

    #119034

    Purpose

    Express soma-localized Voltron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    F74G

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-pCMV-FLPo-pGK-mCl-GP33/66

    Plasmid

    #216481

    Purpose

    Expresses FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover to initiate immunogenic tumor cells in KPFrt mice

    Depositor

    Nikhil Joshi

    Article

    Cheung et al Cancer Cell (2025), doi: 10.1016/j.ccell

    Inserts

    FLPo Recombinase
    mClover3-GP33/66
    

    Use

    Lentiviral

    Promoter

    pCMV and pGK

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK-DTAG-NTERM-GFP

    Plasmid

    #185775

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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