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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 128,594 results for: CIL

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Showing: 9161 - 9180 of 128594 results

  1. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74293-plasmid-map-sequence-id-140421

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  2. pBa-KIF5C 559-tdTomato-FKBP

    Plasmid
    #64211
    Purpose
    KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags
    Depositor
    Gary Banker, Marvin Bentley
    Publication
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kif5c (Kif5c Rat)
    Tags
    FKBP and tdTomato
    Expression
    Mammalian
    Mutation
    motor domain aa1-559
    Promoter
    chicken Beta Actin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64211-plasmid-map-sequence-id-107628

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  10. pCMV-eNme2-T.1-ABE8e

    Plasmid
    #185668
    Purpose
    Expresses eNme2-T.1-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-T.2
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185668-plasmid-map-sequence-id-373131

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  12. pGART-GFP

    Plasmid
    #99106
    Purpose
    Expresses human GART-GFP fusion in mammalian cells
    Depositor
    Stephen Benkovic
    Publication
    An et al Science. 2008 Apr 4;320(5872):103-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GART (GART Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    A65T, L68V, and A72S mutations in GFP gene; kanR/…
    Promoter
    CMV
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99106-plasmid-map-sequence-id-200317

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  15. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68708-plasmid-map-sequence-id-123989

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  16. pTriEx-mPA-GFP-PA-Rac1

    Plasmid
    #22022
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mPA-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  17. pTriEx-PA-Rac1-I539E

    Plasmid
    #22026
    Purpose
    Expression of photoactivatable Rac1 ('lit' state mutant)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains 'lit state' mutatio…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  18. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid
    #44654
    Depositor
    Yoshimi Homma
    Publication
    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    Tags
    FLAG tag and mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    changed Tyr 530 to Phe
    Promoter
    CMV promoter
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44654-plasmid-map-sequence-id-61244

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  19. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Publication
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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Showing: 9161 - 9180 of 128594 results