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pAAV-EF1a-mitoGFP-DIO (AAV2)

Viral prep

#174112-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-EF1a-mitoGFP-DIO (#174112). In addition to the viral particles, you will also receive purified pAAV-EF1a-mitoGFP-DIO plasmid DNA. EF1a-driven, Cre-dependent expression of mitochondria-targeted GFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Maarten Kole

Publication

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Promoter

EF1a

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only
pBsacA

Plasmid

#158650

Purpose

Constitutive transcription of sacA crRNA

Depositor

Wenjing Cui

Insert

sacA crRNA

Use

CRISPR and Synthetic Biology; Shuttle vector baci…

Expression

Bacterial

Promoter

Pveg

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PJEBAN6

Plasmid

#203684

Purpose

Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

Depositor

Tine Rask Licht

Publication

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

DsRed-Express

Expression

Bacterial

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GFPlig

Plasmid

#162223

Purpose

Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBindCY

Plasmid

#158223

Purpose

Expressing CheZ protein in response to blue light

Depositor

Chueh Loo Poh

Publication

Zhang et al J Mol Biol. 2020 May 1;432(10):3137-3148

Insert

CheZ

Use

Synthetic Biology

Tags

YbaQ

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Publication

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Publication

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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miRFP670 - H2A

Plasmid

#176093

Purpose

Nucelear marker

Depositor

Dorus Gadella

Insert

H2A

Expression

Mammalian

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMH0007

Plasmid

#174162

Purpose

UCOE-EF1A-Cas9-BFP

Depositor

Jonathan Weissman

Publication

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Promoter

EF1A

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2xmEGFP

Plasmid

#127799

Purpose

Homo-dimer mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Publication

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP

Expression

Mammalian

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Publication

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

PV IRES

Promoter

SV40

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFUS_A2B

Plasmid

#43850

Depositor

Takashi Yamamoto

Publication

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTN182

Plasmid

#34888

Depositor

Mitsuyasu Hasebe

Publication

Sakakibara et al Evol Dev. 2008 Sep-Oct;10(5):555-66.

Type

Empty backbone

Use

Moss targeting

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PB-TRE-ETV2

Plasmid

#225051

Purpose

PiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2

Depositor

Sean Palecek, Eric Shusta

Publication

Ding et al Biotechnol Bioeng. 2025 Jul;122(7):1914-

Insert

ETS Variant Transcription Factor 2 (ETV2 Human)

Expression

Mammalian

Mutation

Codon Optimized

Promoter

TRE3G

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Publication

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Publication

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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