We narrowed to 13,209 results for: lic

1. pET28A-p53 (1-73) (PPP)

   Plasmid

   #62067

   Purpose

   Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A, P27A

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCMV6-Entry IL36RN R48W

   Plasmid

   #58324

   Purpose

   entry vector for human IL36RN R48W mutant

   Depositor

   Francesca Capon

   Article

   Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

   Insert

   IL36RN (IL36RN Human)
   

   Use

   Entry vector

   Tags

   myc-DDK

   Mutation

   R48W: c.142C>T, p.Arg48Trp

   Promoter

   CMV

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV6-Entry IL36RN P76L

   Plasmid

   #58322

   Purpose

   entry vector for human IL36RN P76L mutant

   Depositor

   Francesca Capon

   Article

   Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

   Insert

   IL36RN (IL36RN Human)
   

   Use

   Entry vector

   Tags

   myc-DDK

   Mutation

   P76L: c.227C>T,p.Pro76Leu​

   Promoter

   CMV

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV6-Entry IL36RN Κ35R

   Plasmid

   #58320

   Purpose

   entry vector for human IL36RN K35R mutant

   Depositor

   Francesca Capon

   Article

   Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

   Insert

   IL36RN (IL36RN Human)
   

   Use

   Entry vector

   Tags

   myc-DDK

   Mutation

   Κ35R: c.104A>G, p.Lys35Arg​

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Pet28a His6-HSPB7 (zebrafish) C117S

   Plasmid

   #110072

   Purpose

   Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 117 to Serine

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pet28a His6-HSPB7 (zebrafish) C49S/C117S

   Plasmid

   #110073

   Purpose

   Express C49S/C117S double mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 49 to Serine, Cysteine 117 to Serine

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCjeCas9

   Plasmid

   #172215

   Purpose

   Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

   Depositor

   John van der Oost

   Article

   Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

   Insert

   Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
   

   Tags

   Histag

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCjedCas9

   Plasmid

   #172216

   Purpose

   Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

   Depositor

   John van der Oost

   Article

   Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

   Insert

   catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
   

   Tags

   Histag

   Mutation

   changed Asp 8 to Ala and His 559 to Ala

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6-Acly

   Plasmid

   #70765

   Purpose

   Expresses mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF6-Acly(S455D)

    Plasmid

    #70768

    Purpose

    Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455D

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF6-Acly(S455A)

    Plasmid

    #70767

    Purpose

    Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455A

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fibronectin-human-EDB in pMAX

    Plasmid

    #120403

    Purpose

    enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

    Depositor

    Inaam Nakchbandi

    Article

    Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

    Insert

    EDB-fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Epac-S-H327

    Plasmid

    #228243

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Epac-S-H328

    Plasmid

    #228244

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Epac-S-H329

    Plasmid

    #228245

    Purpose

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. RN3P-KDM5B-FLAG

    Plasmid

    #86398

    Purpose

    FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

    Depositor

    Wei Xie

    Article

    Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

    Insert

    KDM5B (Kdm5b Mouse)
    

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    Lac

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_TMPO_WT

    Plasmid

    #81818

    Purpose

    Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TMPO (TMPO Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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