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pJ211_WT_hi

Plasmid

#128850

Purpose

High copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

AraBad

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Use

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCO604

Plasmid

#139737

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

H3H

Use

Luciferase; Moclo level 0 component

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-ENK

Plasmid

#184604

Purpose

For expression of a fluorescent sensor for enkephalin in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ENK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJS96

Plasmid

#207588

Purpose

GFP-TonBH20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonBH20A

Use

Tags

Expression

Bacterial

Mutation

H20A

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Clathrin-mhYFP

Plasmid

#186527

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Clathrin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBbS15k-RFP

Plasmid

#126194

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCO486

Plasmid

#139729

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A_35S Short_TMV 5':AsNpga:tAtug7

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP

Plasmid

#64887

Purpose

Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

Depositor

Tomomi Ichinose

Article

Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

Insert

iRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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DGK gamma

Plasmid

#35408

Depositor

Stephen Prescott, Robert Lefkowitz

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK gamma (DGKG Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pBbA19a-RFP

Plasmid

#126101

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet

Plasmid

#104311

Purpose

codes for a fusion of fluorescent protein mGarnet and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

H2B-mGarnet

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-AGT

Plasmid

#184598

Purpose

For expression of a fluorescent sensor for angiotensin II in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AGT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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CeNL-H2B_pcDNA3

Plasmid

#89511

Purpose

Cyan color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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ChlorON-2

Plasmid

#193478

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-2

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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DGK delta

Plasmid

#35406

Depositor

Stephen Prescott, Robert Lefkowitz

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK delta (DGKD Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJB066

Plasmid

#98913

Purpose

Express FtsZ tagged with pAmCherry1 in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Use

Tags

pAmCherry1

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mClover3-EC18157

Plasmid

#137076

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GGvT

Plasmid

#87362

Purpose

Expresses GGvT, a tandem homodimer green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GGvT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P366

Plasmid

#139722

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A''_pSlRBCS2:AsNpga:tAtuG7

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

SlRBCS2

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Use

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCO487

Plasmid

#139730

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_B_AtUbi10:NnH3H:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCO488

Plasmid

#139731

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_CmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-EB

Plasmid

#28297

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (Nherf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains only the EB domain aa 262-355 (deletion …

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pRSM42

Plasmid

#113942

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRGECO1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSG26(gfcD)

Plasmid

#176198

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-Green Falcan0.3

Plasmid

#199704

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan0.3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-Green Falcan10

Plasmid

#199707

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-RDSmCherry1

Plasmid

#89987

Purpose

Red-Shifted variants of red fluorescent protein mCherry

Depositor

Robert Campbell

Article

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

RDSmCherry1

Use

Tags

6xHis-T7-Xpress

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-CCK

Plasmid

#184602

Purpose

For expression of a fluorescent sensor for CCK in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-CCK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-Green Falcan3

Plasmid

#199706

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1801 - 1840 of 21952 results

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