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TagBFP(S2)3(S4)3-gs

Plasmid

#228068

Purpose

Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Expression

Insect

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C

Plasmid

#80932

Purpose

Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Smad4-Flag

Plasmid

#37041

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

Smad4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HkCas12a-2AeGFP

Plasmid

#123634

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

HkCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)3(S4)3-SAH

Plasmid

#228069

Purpose

Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)2(S4)2-gs

Plasmid

#228072

Purpose

Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPyCAG-tagBFP-3xNLS-IRES-Hph

Plasmid

#183608

Purpose

Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tagBFP-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-2AeGFP

Plasmid

#123638

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

AsCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18-H1 RNAi

Plasmid

#87355

Purpose

RNAi expression vector based on H1 promoter and pUC18 vector backbone

Depositor

Saroj Mathupala

Article

Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

Insert

H1 RNA Promoter from U87MG glioma cells

Use

RNAi; Shrna expression vector

Expression

Mammalian

Promoter

H1 RNA

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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NavBp

Plasmid

#100014

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NavBp

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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NsvBa

Plasmid

#100015

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NsvBa

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-PxCas12a-2AeGFP

Plasmid

#123637

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PxCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-Max-T2A-EGFP

Plasmid

#188268

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-HiFi-T2A-EGFP

Plasmid

#188269

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-HiFi

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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