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Showing: 901 - 920 of 52672 results

  1. INT_GFPsp

    Plasmid
    #212144
    Purpose
    Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212144-plasmid-map-sequence-id-420757

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  2. INT_GFPsp_CamRcargo

    Plasmid
    #212143
    Purpose
    Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212143-plasmid-map-sequence-id-420641

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  3. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid
    #169403
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169403-plasmid-map-sequence-id-336889

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  4. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid
    #169404
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169404-plasmid-map-sequence-id-335424

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  5. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid
    #159906
    Purpose
    Mutagenesis of Dcc
    Depositor
    Larry Zweifel
    Insert
    Dcc (Dcc Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159906-plasmid-map-sequence-id-317312

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  6. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  8. pNR2F1.1.0-gDNA

    Plasmid
    #112484
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1
    Depositor
    Kevin White
    Insert
    NR2F1 (NR2F1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112484-plasmid-map-sequence-id-230521

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  9. pELF4.1.0-gDNA

    Plasmid
    #112467
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4
    Depositor
    Kevin White
    Insert
    ELF4 (ELF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112467-plasmid-map-sequence-id-230497

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  10. pCMV-CreEGFP

    Plasmid
    #165140
    Purpose
    Expression of Cre recombinase fused with EGFP in C-terminal
    Depositor
    David Threadgill, Daekee Lee
    Insert
    Cre recombinase
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165140-plasmid-map-sequence-id-326583

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  11. PB-CMV-MCS-Puro

    Plasmid
    #219794
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219794-plasmid-map-sequence-id-441249

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  12. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  13. pBAD-pHScarlet

    Plasmid
    #191258
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet
    Depositor
    Robert Campbell
    Insert
    pHScarlet
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191258-plasmid-map-sequence-id-374867

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  14. pUPD2 CBS4 (GB2718)

    Plasmid
    #160601
    Purpose
    Copper dependent CUP2 binding DNA sequence
    Depositor
    Diego Orzaez
    Insert
    CBS4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160601-plasmid-map-sequence-id-322066

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  15. pUPD2 GFP-CT (GB2238)

    Plasmid
    #160592
    Purpose
    GFP for C-terminal fusions.
    Depositor
    Diego Orzaez
    Insert
    GFP-CT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160592-plasmid-map-sequence-id-322074

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  16. AV0-BWS-2.0-mC-G-CL3B

    Plasmid
    #124975
    Purpose
    evaluate autophagy
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-GFP-LC3B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124975-plasmid-map-sequence-id-243955

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  17. RHPMP139 (pLifeAct mEOS3.1)

    Plasmid
    #88839
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    mEOS3.1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88839-plasmid-map-sequence-id-177489

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  18. RHPMP140 (pLifeAct mOrange2)

    Plasmid
    #88840
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88840-plasmid-map-sequence-id-177491

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  19. pUC19-U6-terminator (SL695)

    Plasmid
    #133961
    Purpose
    pUC19 plasmid with U6 promoter followed by a poly-T termination signal
    Depositor
    Keith Joung
    Insert
    U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133961-plasmid-map-sequence-id-264466

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  20. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid
    #113837
    Purpose
    Monomeric TagBFP2 and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113837-plasmid-map-sequence-id-222246

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Showing: 901 - 920 of 52672 results