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pAc5.1B-EGFP-DmGW182-DUF-Cterm_L

Plasmid

#146832

Purpose

Insect Expression of DmGW182-DUF-Cterm

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-DUF-Cterm (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-DUF-RBD_L

Plasmid

#146833

Purpose

Insect Expression of DmGW182-DUF-RBD

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-DUF-RBD (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1_1250-1723-F1370A_K

Plasmid

#146745

Purpose

Mammalian Expression of HsTNRC6Biso1_1250-1723-F1370A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1250-1723-F1370A (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-siRNAres_I

Plasmid

#146543

Purpose

Mammalian Expression of HsTNRC6Biso1-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1-siRNAres (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOAD eIF4G1 (1-341)

Plasmid

#196833

Purpose

Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

eIF4G1 (TIF4631 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

amino acids 1-341

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2 Pab1 WT (123-204)

Plasmid

#196834

Purpose

Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

Pab1 (PAB1 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

123-204 amino acids

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2 Pab1 E181R (123-204)

Plasmid

#196835

Purpose

Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

Pab1 (PAB1 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

123-204 amino acids & E181R

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSA

Plasmid

#195207

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340A

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

S162/339/340D

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R221C SPOP 28-359

Plasmid

#194116

Purpose

R221C His SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP R221C (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R221C

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRBx2

Plasmid

#193569

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PH-mCherry-FRBx2

Plasmid

#193570

Purpose

Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and mCherry

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564A

Plasmid

#191978

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564A (Mfn1 Mouse)

Expression

Mammalian

Mutation

T564A

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564D

Plasmid

#191979

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564D (Mfn1 Mouse)

Expression

Mammalian

Mutation

T564D

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase{dead}

Plasmid

#187869

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase(dead)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase{dead}

Tags

GFP

Expression

Bacterial

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::axed{sgRNA})

Plasmid

#187883

Purpose

Gal4/UAS sgRNA expression targeting axed

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting axed

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::hiw{sgRNA})

Plasmid

#187884

Purpose

Gal4/UAS sgRNA expression targeting hiw

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting hiw

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::dsarm{sgRNA})

Plasmid

#187885

Purpose

Gal4/UAS sgRNA expression targeting dsarm

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting dSarm

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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