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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,111 results for: grna

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Showing: 9181 - 9200 of 16111 results

  5. pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry

    Plasmid
    #254862
    Purpose
    peCHYRON destination vector expressing IPTG-inducible B-to-A pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254862-plasmid-map-sequence-id-512990

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  6. pTBL3439 MTK_PBdest lacO-hU6-AtoBpegRNA-LacI-mCherry

    Plasmid
    #254861
    Purpose
    peCHYRON destination vector expressing IPTG-inducible A-to-B pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_AtoB_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254861-plasmid-map-sequence-id-512989

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  9. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  10. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  16. pDual_dsCas9_Venus_hs_NQO1

    Plasmid
    #214684
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1
    Depositor
    Kevin Janes
    Insert
    dgRNA_NQO1 (NQO1 Human)
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214684-plasmid-map-sequence-id-428771

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  17. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

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  18. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

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  20. pMSX2.1.0-gDNA

    Plasmid
    #113793
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MSX2
    Depositor
    Kevin White
    Insert
    MSX2 (MSX2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113793-plasmid-map-sequence-id-241255

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Showing: 9181 - 9200 of 16111 results