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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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S-HBsAg

Plasmid

#103013

Purpose

Expresses HBV Small envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Small envelope protein

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM9121_GFP11

Plasmid

#153508

Purpose

GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Green Glifon50

Plasmid

#126206

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon50

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHelp

Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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Green Glifon4000

Plasmid

#126208

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon4000

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

Plasmid

#60660

Purpose

Flp-dependent expression of mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX-mNaChBacMut-T2A-tdTomato

Use

AAV

Mutation

E191K

Promoter

EF1α

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pYMA1_SidK_mCH

Plasmid

#184906

Purpose

Legionella pneumophila SidK (1-278) - mCherry

Depositor

John Rubinstein

Article

Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

Insert

SidK (1-278) (sidK Legionella pneumophila)

Tags

mCherry

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-Fluc

Plasmid

#183474

Purpose

This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

Firefly luciferase

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eNpHR3.0-mRuby2-ST

Plasmid

#109134

Purpose

Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eNpHR3.0-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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