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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,964 results for: nin

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Showing: 9201 - 9220 of 66964 results

  2. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Publication
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  3. pDisplay-NE2m-IRES-mCherry-CAAX

    Plasmid
    #208690
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells
    Depositor
    Yulong Li
    Publication
    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208690-plasmid-map-sequence-id-412239

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  9. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  11. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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  13. NYESO beta into TRBC1 HDRT Source (pTR 277)

    Plasmid
    #112024
    Purpose
    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NYESO beta into TRBC1 HDRT
    Use
    CRISPR and Synthetic Biology
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    112024-plasmid-map-sequence-id-215922

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  15. pEM705 Gsi (Cys)

    Plasmid
    #109373
    Purpose
    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gsi chimera
    Tags
    None
    Expression
    Mammalian
    Mutation
    Cys at position 391 (Pertussis Toxin Sensitive)
    Promoter
    CAG
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109373-plasmid-map-sequence-id-206232

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  17. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87260-plasmid-map-sequence-id-176554

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  19. pcDNA3.1-MAP3K5 (R256C)

    Plasmid
    #47105
    Purpose
    Expresses mutant (R256C) human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K5 (MAP3K5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed arginine 256 to cysteine
    Promoter
    CMV
    Available since
    Oct. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. SMARCA4

    Plasmid
    #39116
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA4 (SMARCA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 9201 - 9220 of 66964 results