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pCGN-HCF-1-C600

Plasmid

#92324

Purpose

C-term of HCF-1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Insert

HCF-1 C-term 1436-2035 (HCFC1 Human)

Tags

C-myc (EQKLISEED) and HA

Expression

Mammalian

Mutation

AA 1436-2035

Promoter

CMV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

human SYN1

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3myc-PR-LBD

Plasmid

#89113

Purpose

mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor ligand binding domain (PGR Human)

Tags

myc

Expression

Mammalian

Mutation

contains Ligand Binding Domain (632-933)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pSB165 - pL1R3_pMAS::rLUC-I::tMAS

Plasmid

#123198

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pMAS::rLUC-I::tMAS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-173

Plasmid

#104193

Purpose

Expresses human AR aa 1-173 in pGEX-5x-1 bacterial expression vector with GST

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-173 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 3KR

Plasmid

#78841

Purpose

To overexpress RIPK1 3KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Mutation

K571R, K604R, K627R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255, R155A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K316R

Plasmid

#78837

Purpose

To overexpress RIPK1 K316R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Mutation

K316R

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pEBTetBl-CLIP-plk4

Plasmid

#136837

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-plk4 (PLK4 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-2-429

Plasmid

#89133

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2009 Dec 11;284(50):34793-8

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

contains aa 2-429

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
YFP-Rac1(H40,L61)

Plasmid

#11403

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine. Th…

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 2KR(89,501)

Plasmid

#78812

Purpose

To overexpress RIPK3 2KR(89,501) in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K89R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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B0032m_BC

Plasmid

#66020

Purpose

MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pJ02B2Gm_EF

Plasmid

#66064

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3 4KR

Plasmid

#78821

Purpose

To overexpress RIPK3 4KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only