We narrowed to 9,647 results for: bli

1. Human b2 nicotinic subunit crystallization construct

   Plasmid

   #85842

   Purpose

   pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

   Insert

   Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   C-terminal StrepII tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ-ABE-SpRYn-Act3.0

   Plasmid

   #178959

   Purpose

   It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF3486

   Plasmid

   #144962

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Human a3 nicotinic receptor subunit EM construct

   Plasmid

   #163960

   Purpose

   pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human alpha3 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Asn348-Ser402 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-TRE-flex-Clover

   Plasmid

   #135177

   Purpose

   AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

   Inserts

   Clover
   TRE3G
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   a variant of GFP

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF2032

   Plasmid

   #142907

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIBN-TetR

   Plasmid

   #103813

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-TetR (CIB1, tetR Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LIC-Z

   Plasmid

   #153543

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEBG-IFNAR2(265-515)

    Plasmid

    #12437

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    IFNAR2 a.a. 265-515 (IFNAR2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMinDis.X513-iNicSnFR3b-(V7)-PM

    Plasmid

    #125124

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)-PM
    

    Tags

    Ig-K-Chain and membrane anchoring protein-PDGFR

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

    Plasmid

    #121839

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter mir16-1.2

    Plasmid

    #46681

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.2 (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ABEmax-Puro V2

    Plasmid

    #226955

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJRoC30_Th3.1

    Plasmid

    #188065

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 4 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRoC30_Th3.7

    Plasmid

    #188066

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRoC30_Th3.10

    Plasmid

    #188067

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCrhaB2_ApyOAHIDS

    Plasmid

    #226246

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCrhaB2_ApyOAHID

    Plasmid

    #226245

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge