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pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

Plasmid

#55219

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pPP444

Plasmid

#158802

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone)

Plasmid

#238183

Purpose

Lentiviral empty vector

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec31A

Plasmid

#66612

Purpose

CFP fusion of Sec31A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Tags

ECFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Promoter

T7

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1171 FIREmate-ECFP-Giantin

Plasmid

#205166

Purpose

Expresses FIREmate-ECFP-Giantin in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Giantin

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

SNAP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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