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pET29b-pld

Plasmid

#128795

Purpose

expresses E. coli PLD in bacteria

Depositor

Emily Balskus

Article

Chittim et al Nat Microbiol. 2019 Jan;4(1):155-163. do

Insert

Phospholipase D

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIC242

Plasmid

#44432

Depositor

Iain Cheeseman, Arshad Desai

Article

Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Insert

Mob1A (MOB1A Human)

Use

Retroviral

Tags

GFP, s peptide, and tev

Expression

Mammalian

Mutation

F5L, K15R and G212R

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LZF1723: mI12b-EGFP

Plasmid

#104123

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mI12b

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVd2-mCherry-Dr-TrkA

Plasmid

#127957

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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SI11-Sema4c

Plasmid

#46301

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Sema4c mouse monoclonal antibody (sc:d411 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCfB8818

Plasmid

#126909

Purpose

Template for PCR amplification of HMG1from Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

YlHMG1

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-31 site

Plasmid

#25021

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-31 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
BB1_O_34_AMA1_2.8

Plasmid

#90293

Purpose

BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

AMA1_2.8

Expression

Bacterial

Mutation

it is a truncated version of AMA1 (5.3 kb)

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pERB111

Plasmid

#58281

Purpose

constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

empty miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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psiCheck2 3xUTR

Plasmid

#40762

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Expression

Bacterial

Mutation

C114S, L118S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pGBW-m4046447

Plasmid

#145387

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGALT-EGFP-IRES-puro2b

Plasmid

#26463

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pGalT

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R4

Plasmid

#124211

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR04

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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SI7-NCAM2

Plasmid

#36142

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

NCAM2 mouse monoclonal antibody (ncam2 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
SI8-IGSF21b

Plasmid

#36143

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

IGSF21b mouse monoclonal antibody (igsf21b Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only