172,845 results

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EGFP-Vimentin-7

Plasmid

#56439

Purpose

Localization: Intermediate Filaments, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PKC alpha WT

Plasmid

#21232

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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hCRISPRi_dual_1_2

Pooled Library

#187246

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
pHR_PGK_BFP_2A-mIL10

Plasmid

#247507

Purpose

Expresses mouse IL-10 and BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

mouse IL-10 and BFP constitutive (Il10 Synthetic, Mouse)

Use

Lentiviral

Tags

bfp

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CRISPi Kit

Plasmid Kit

#1000000136

Purpose

Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.

Depositor

Brigitte Gasser

Application

Genome Editing

Vector Type

Yeast Expression

Editing Type

CRISPR

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only
Cavb3

Plasmid

#26574

Depositor

Diane Lipscombe

Insert

calcium channel beta subunit-III (Cacnb3 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205317

Purpose

All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISYmu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Mito-Dendra2

Plasmid

#238077

Purpose

Expresses photoactivable fluorescent reporter for visualizing mitochondrial network

Depositor

Partha Mondal

Insert

Dendra2

Tags

Mito

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#209654

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-BMPR2a

Plasmid

#207616

Purpose

Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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