We narrowed to 19,051 results for: Tec

1. pLCHKO_MDM4_exon_deletion_3

   Plasmid

   #155075

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-707pUC19-tNGFR-P2A-TTC37

   Plasmid

   #186122

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   TTC37 (SKIC3 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNAS1b split mCherry NEDD4 WW2 pos ctrl

   Plasmid

   #112029

   Purpose

   Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
   

   Tags

   Split mCherry

   Expression

   Bacterial

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX018

   Plasmid

   #167144

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal luciferase, nnLuz
   

   Use

   Luciferase and Synthetic Biology

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. NME2 gRNA (BRDN0001487089)

   Plasmid

   #77932

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NME2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NME1-NME2,NME2 (NME2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIK3CB gRNA (BRDN0001147768)

   Plasmid

   #76194

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3CB

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLM3727

   Plasmid

   #49961

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CD HB-5 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

   Plasmid

   #105644

   Purpose

   Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   CMV:ikk1FL(C178A)-tdTomato

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

9. PNKP gRNA (BRDN0001147028)

   Plasmid

   #77162

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PNKP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PNKP (PNKP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRKRA gRNA (BRDN0001146443)

    Plasmid

    #76299

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKRA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKRA (PRKRA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SGK3 gRNA (BRDN0001146019)

    Plasmid

    #75540

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    C8orf44-SGK3,SGK3 (SGK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. SGK3 gRNA (BRDN0001146281)

    Plasmid

    #75541

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    C8orf44-SGK3,SGK3 (SGK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RPS6KA5 gRNA (BRDN0001145521)

    Plasmid

    #77372

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PA-mCit-NL

    Plasmid

    #113452

    Purpose

    Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    hUBbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZAP70 gRNA (BRDN0001487050)

    Plasmid

    #77956

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAP70

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZAP70 gRNA (BRDN0001145290)

    Plasmid

    #77957

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAP70

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TTBK2 gRNA (BRDN0001487117)

    Plasmid

    #77969

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTBK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTBK2 (TTBK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TTBK1 gRNA (BRDN0001145252)

    Plasmid

    #75801

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTBK1 (TTBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TTBK1 gRNA (BRDN0001148007)

    Plasmid

    #75803

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTBK1 (TTBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge