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p7134 pHAGE-P-CMVt-N-HA-GAW-WDR92

Plasmid

#100161

Purpose

Expresses WDR92

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

WDR92 (WDR92 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pME-CD44a

Plasmid

#109575

Purpose

Multisite gateway vector for 5' tagging with CD44a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44a (cd44a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-CD44a-mKate2

Plasmid

#109523

Purpose

Muscle specific zebrafish expression of mKate2 fused to CD44a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44a (cd44a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-CD44b-mKate2

Plasmid

#109524

Purpose

Muscle specific zebrafish expression of mKate2 fused to CD44b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44b (cd44b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-PK1Dexp

Plasmid

#130834

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV Cyclin D2-long-mut

Plasmid

#21657

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

mutated miR-15/16 and let-7 binding sites

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
AAV-Y-GECO1

Plasmid

#55767

Purpose

Express Y-GECO1 in neurons

Depositor

Robert Campbell

Article

Zhao et al Integr Biol (Camb). 2014 May 19.

Insert

Y-GECO1.0

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSG 417

Plasmid

#63864

Purpose

Detect translation initiation from AUU start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUU start codon

Promoter

PtacI

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-VASP-5

Plasmid

#55513

Purpose

Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VASP

Tags

mTFP1

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Smp_112090-Cd4-bio-His

Plasmid

#120662

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_112090 (Smp_112090 Schistosoma mansoni)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
SRF 1-133 pGEX4T1

Plasmid

#121101

Purpose

Expression of SRF fragment as a GST fusion protein

Depositor

Paul Herring

Article

Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

Insert

SRF (SRF Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 1-133

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1252_TetGFP(Hyg)

Plasmid

#118993

Purpose

TetOFF controlled GFP-tagging vector with Hygromycin selection

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression (in magnaporthe oryzae)

Promoter

Ustilago maydis Mfa1 basal promoter with six tetO…

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rNanog-pgk-hph

Plasmid

#74917

Purpose

Mammalian expression of rNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Rat)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJLI-SUP35 (delta)2-5

Plasmid

#1138

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted, but …

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pRSET FLII12Pglu-600uDelta4

Plasmid

#13567

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 4aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pY128

Plasmid

#127713

Purpose

Level 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSG 416

Plasmid

#63863

Purpose

Detect translation initiation from AUA start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUA start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only