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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

SH4

Tags

FRB-HA-EBFP2

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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BLA_CAT_K136_GPG_LOV2

Plasmid

#231575

Purpose

This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

CAT_ carrying an AsLOV2 insertion behind K136

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSFS2A-CaKan

Plasmid

#189565

Purpose

Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

CaKan

Expression

Yeast

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-48-3xFLAG

Plasmid

#130675

Purpose

Expresses full-length human SMCHD1 with a C-terminal 3x FLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCN38-yfp

Plasmid

#227637

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207847

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P

Plasmid

#207846

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJB007

Plasmid

#98995

Purpose

Express FtsI tagged with tagRFP-t in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsI

Tags

TagRFP-T

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBIG2ABC-eIF3Δbgi

Plasmid

#240043

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d, eIF3m, eIF3a and eIF3c expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m, a, c

Tags

eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLG008 (Ec78 retron)

Plasmid

#157886

Purpose

Expresses the Ec78 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec78 retron

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Expression

Bacterial

Promoter

Lambda promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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