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Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

Depositor

Bradley Bernstein, Gabriel Griffin, Robert Manguso

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
Barcode Library 0 (BCL0)

Pooled Library

#179851

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 1 (BCL1)

Pooled Library

#179852

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only
pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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P3-Lenti-dCas9-Tet1-GFP

Plasmid

#190729

Purpose

Lentiviral construct to express spdCas9-Tet1-GFP.

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

SV40 NLS and T2A-EGFP

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

UbC

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-307_LgBiT

Plasmid

#191211

Purpose

Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

LgBiT (large bit)

Use

Lentiviral and Luciferase

Mutation

AB030246.1 (mutated in PMID 26569370)

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-10xHis

Plasmid

#194178

Purpose

Expresses MPP8-10xHis tagged

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn CaBLAM

Plasmid

#244227

Purpose

Bioluminescent reporter for calcium signaling in neurons

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-dTomato

Plasmid

#102631

Purpose

In vitro expression of tdTomato from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

tdTomato

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP4e-Kv-WPRE

Plasmid

#201031

Purpose

Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 711-1034

Plasmid

#75141

Purpose

Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 711-1034

Promoter

CMV

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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