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We narrowed to 13,988 results for: mpl

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Showing: 9361 - 9380 of 13988 results

  3. pLuc-Optop38i-lsm

    Plasmid
    #89747
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89747-plasmid-map-sequence-id-192491

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  4. pLuc-OptoJNKi-lsm

    Plasmid
    #89746
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89746-plasmid-map-sequence-id-192490

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  5. pLuc-OptoJNKi-dsm

    Plasmid
    #89745
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89745-plasmid-map-sequence-id-192487

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  6. pGAPTrap-Clover-Muro

    Plasmid
    #82334
    Purpose
    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Clover-T2AMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    82334-plasmid-map-sequence-id-156570

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  7. pGAPTrap-eGFP-IRESMeo

    Plasmid
    #82502
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMeo
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82502-plasmid-map-sequence-id-156575

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  9. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72427-plasmid-map-sequence-id-137744

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  10. G6f-bio-His

    Plasmid
    #53342
    Purpose
    Expresses full-length Lymphocyte antigen 6 complex locus protein G6f precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    G6f (LY6G6F Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53342-plasmid-map-sequence-id-83072

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  15. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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  17. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  19. pD649-HAsp-ULBP1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156597
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ULBP1 (ULBP1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156597-plasmid-map-sequence-id-420967

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  20. pD649-HAsp-SEMA4C-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156935
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA4C (SEMA4C Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156935-plasmid-map-sequence-id-412904

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Showing: 9361 - 9380 of 13988 results