We narrowed to 16,047 results for: Coli

1. EcYajL pET15b

   Plasmid

   #60716

   Purpose

   Recombinant protein expression of YajL

   Depositor

   Mark Wilson

   Article

   Wilson et al J Mol Biol. 2005 Oct 28;353(3):678-91. E

   Insert

   YajL
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAW212

   Plasmid

   #113234

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GGTAG->CCATC

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEL1868

   Plasmid

   #170417

   Purpose

   Genetic complementation plasmid of LetAB with LetB mutant L599N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(L599N)

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEcCas5

   Plasmid

   #170090

   Purpose

   expresses E. coli type I-E Cas5

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEcCas7

   Plasmid

   #170092

   Purpose

   expresses E. coli type I-E Cas7

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas7
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcCas8

   Plasmid

   #170093

   Purpose

   expresses E. coli type I-E Cas8

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEcCse2

   Plasmid

   #170094

   Purpose

   expresses E. coli type I-E Cse2

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cse2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACYCDuet_DL121_WTTS

   Plasmid

   #171954

   Purpose

   Selection vector for chimeric fusion of ecDHFR and asLOV2.

   Depositor

   Kimberly Reynolds

   Article

   McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

   Inserts

   Fusion of ecDHFR and asLOV2 at position 121 of DHFR
   Thymidylate Synthase
   

   Use

   Tags

   8-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW228

   Plasmid

   #113242

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   TTGGTAG->tAggtag

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW211

    Plasmid

    #113233

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->AACGA

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCASCADE-gltA1-gltA2

    Plasmid

    #71348

    Purpose

    gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    gltA1-gltA2 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHB1

    Plasmid

    #212153

    Purpose

    For PHB production in E. coli (PT7-pMB1).

    Depositor

    Kang Zhou

    Insert

    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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