We narrowed to 32,439 results for: Eng;
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Plasmid#129105PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone productionDepositorInsertBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
ExpressionBacterialAvailable SinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSM-de-Pfba1-PYC-Trpl15a
Plasmid#127738PurposeYeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyExpressionYeastPromoterPfba1Available SinceJan. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC_delta ald1
Plasmid#126708Purposevector for knocking out ald1 in Komagataella phaffiiDepositorInsertHIS4
ExpressionYeastPromoterHisAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ab-Psptef1-NAT-Ttip1
Plasmid#127747PurposeYeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.DepositorInsertnourseothricin N-acetyltransferase
UseSynthetic BiologyExpressionYeastPromoterPsptef1Available SinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFNC-5
Plasmid#233297PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-6
Plasmid#227669PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC022 (∆phcA)
Plasmid#226200PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC004 (∆phaCAB)
Plasmid#226198PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 15, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
(J) crtYB(K) G3 (SBE117)
Plasmid#194996PurposeRhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3DepositorInsert(J) crtYB(K)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(G) crtI (H) G2 (SBE116)
Plasmid#194995PurposeRhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2DepositorInsert(G) crtI (H)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(D) crtE (E)_G1 (SBE115)
Plasmid#194994PurposeRhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1DepositorInsert(D) crtE (E)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA2ScIfm
Plasmid#193786PurposeExpresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyExpressionBacterialPromoterPLTATAA synthetic promoter carrying binding sites…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA1cI-T3
Plasmid#193789PurposeExpresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertTruncated version of Lambda CI, named T3, where the first two codons are deleted
UseSynthetic BiologyExpressionBacterialPromoterPLTetO-1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH666
Plasmid#169610PurposeTier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
ExpressionMammalianPromotertetO7-PCMVmin / PPGK / PPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH664
Plasmid#169608PurposeTier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
ExpressionMammalianPromotertetO7-PCMVmin / PPGK / PPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2343_Tier3(SB)-YPet_Blast
Plasmid#169641PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and BlastR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2344_Tier3(SB)-mRuby2_Blast
Plasmid#169642PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and BlastR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64
Plasmid#169597PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).DepositorInsertdead S.pyogenes Cas9 - VP64 fusion
ExpressionMammalianPromoterPhCMVAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2339_Tier3(SB)-Ruby_Puro
Plasmid#169637PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only