We narrowed to 2,167 results for: PAM

1. AA025

   Plasmid

   #216005

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-NG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA180

   Plasmid

   #216023

   Purpose

   Fragmid fragment: (Cas protein) NGRR(T) PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA027

   Plasmid

   #216007

   Purpose

   Fragmid fragment: (Cas protein) nearly PAM-less

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpRY_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA026

   Plasmid

   #216006

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JAM1-CD4d3+4-bio

   Plasmid

   #73110

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding JAM1 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   JAM1 (F11R Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPT-/mEGFP-1T-IRES-mCherry

   Plasmid

   #190190

   Purpose

   Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Mutation

   changed EGFP Kozak sequence inserting a C>T mo…

   Promoter

   EIF1-short

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA007

   Plasmid

   #216002

   Purpose

   Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET28-FnCas12a-EP16

   Plasmid

   #183636

   Purpose

   PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

   Depositor

   Yunzi Luo

   Article

   Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

   Insert

   FnCas12a-EP16
   

   Use

   CRISPR

   Tags

   6×His Tag

   Expression

   Bacterial

   Mutation

   N607R, K613V, N617R, K180S, K660R, D616N

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV_Npu-saBE3.9max_KKH C-terminal

   Plasmid

   #137181

   Purpose

   Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-saBE3.9max_KKH_C-terminal
   

   Expression

   Mammalian

   Mutation

   E782K;N968K;R1015H conferring recognition of NNNR…

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEffector-LaTranC-CRISPR RNA

    Plasmid

    #246928

    Purpose

    for E.coli plasmid interference and PAM depletion assays

    Depositor

    Caixia Gao

    Article

    Jin et al Cell. 2025 Sep 29:S0092-8674(25)01035-9.

    Insert

    LaTranC
    

    Use

    Unspecified

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-2T-FRB-W2027F

    Plasmid

    #73341

    Purpose

    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; W2027F

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-2T FRB Y2105A

    Plasmid

    #68726

    Purpose

    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; Y2105A

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBS-I-Sce1-slc6a3[11kb]:egfp

    Plasmid

    #53240

    Purpose

    allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT-GFP
    

    Use

    Cloning

    Tags

    GFP

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA028

    Plasmid

    #200419

    Purpose

    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Type

    Empty backbone

    Tags

    PAmCherry1

    Expression

    Bacterial

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIA033

    Plasmid

    #200420

    Purpose

    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Type

    Empty backbone

    Tags

    bsPAmCherry

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 luciferase

    Plasmid

    #26612

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    firefly luciferase
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pSR1054

    Plasmid

    #208740

    Purpose

    Characterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PAmy
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PAmy

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-TOP luciferase

    Plasmid

    #26611

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    eEF2 5'UTR
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-2T FRB

    Plasmid

    #26607

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    FRB domain only (aa 2015-2114)

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. slc6a3 probe

    Plasmid

    #53764

    Purpose

    cRNA probe to detect the slc6a3 transcript

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT
    

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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