Addgene

We narrowed to 76,091 results for: TRO

Showing: 921 - 940 of 76091 results

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pVITRO1-SS-131

Plasmid

#68736

Purpose

ZF8-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF8-VP64
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-132

Plasmid

#68729

Purpose

ZF1-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF1-VP64
mCherry

Use

Tags

FLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-THAP11-A

Plasmid

#36993

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-THAP11-C

Plasmid

#36994

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-THAP11-E

Plasmid

#36995

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

Plasmid

#22131

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-EGFP-Frame0-Parental-Minicircle

Plasmid

#216124

Purpose

Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his

Plasmid

#24055

Depositor

Jonathan Backer

Article

Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Insert

PIK3C3 and PIK3R4 (PIK3C3, PIK3R4 Human)

Use

Bicistronic

Tags

Myc (hVps34) and V5/his (hVps15)

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pM123: pAAV-EFS-CasRx-control presgRNA

Plasmid

#166871

Purpose

AAV vector for expressing CasRx and control presgRNA for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-control presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179120

Purpose

Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

control sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mPGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

Plasmid

#227004

Purpose

AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

Insert

alpha synuclein A53T mutant (SNCA Synthetic, Human)

Use

AAV

Tags

Expression

Mutation

A53T

Promoter

CMVenh synapsin

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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