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pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-ArCas12a-2AeGFP

Plasmid

#123632

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

ArCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-LpCas12a-2AeGFP

Plasmid

#123635

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

LpCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12a-2AeGFP

Plasmid

#123633

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

BsCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-PrCas12a-2AeGFP

Plasmid

#123636

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PrCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(D977A)-2AeGFP

Plasmid

#121962

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

D977A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 30R3-1

Plasmid

#120304

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

30R3-1, Gli3

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTL52_pyCAG-s36GFPHaloTag-IRES-mCherry3NLS-polyA

Plasmid

#223544

Purpose

Minimal PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-HaloTag, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA

Plasmid

#223545

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Cyclone-Cre C-DYK

Plasmid

#247496

Purpose

Acyclovir-regulated Cre

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

Cre with Cyclone insertion

Use

Cre/Lox and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Mutation

Cyclone was inserted after Methionine300 of Cre t…

Promoter

CMV

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SlmiR482bTS-B/c

Plasmid

#234368

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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(P6f)3(P14f)3-gs

Plasmid

#228082

Purpose

Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

P6f, P14f

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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(S1h-S3h)3-gs

Plasmid

#228078

Purpose

Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

S1h, S3h

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)8-gs

Plasmid

#228086

Purpose

Mammalian expression of coiled coil segments (S2h)x8, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2-S4)1-gs

Plasmid

#228074

Purpose

Mammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1-S3)1-SAH

Plasmid

#228073

Purpose

Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCitrine, S1, S3

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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