We narrowed to 30,743 results for: SHE

1. tcTRP24sub8

   Plasmid

   #175796

   Purpose

   de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub8
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. tcTRP24sub8_SS

   Plasmid

   #175798

   Purpose

   de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub8_SS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tcTRP24sub8_SH2sub6

   Plasmid

   #175799

   Purpose

   de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub8_SH2sub6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tcTRP9sub3

   Plasmid

   #175794

   Purpose

   de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP9sub3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tcTRP24sub6

   Plasmid

   #175795

   Purpose

   de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

   Plasmid

   #168770

   Purpose

   B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting; Dre / rox

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3-HA PSPC1 Y523F

   Plasmid

   #124373

   Purpose

   Expresses PSPC1 Y523F in mammalian cells

   Depositor

   Yuh-Shan Jou

   Article

   Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

   Insert

   PSPC1 (PSPC1 Human)
   

   Use

   Tags

   HA and HA tag

   Expression

   Mammalian

   Mutation

   Y523F

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

   Plasmid

   #141452

   Purpose

   B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Mouse Targeting; Dre / rox

   Tags

   C-terminal fusion of T2A-TagRFPT-dre-PBD*

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK2 3'UTR GAC

   Plasmid

   #110401

   Purpose

   Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19 KGA HA5'-mKO2-3'

    Plasmid

    #110406

    Purpose

    Homology donor for glutaminase KGA isoform knock-in, mKO2

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-Myosin7B-sgRNA

    Plasmid

    #154861

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    MYO7B sgRNA (MYO7B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET20b-YbAnbu

    Plasmid

    #129637

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbu
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET20b-YbAnbuT1A

    Plasmid

    #129638

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbuT1A
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    T1A, V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCool-human TSC1 (939-992)

    Plasmid

    #132618

    Purpose

    expresses human TSC1 (939-992) in E. coli

    Depositor

    Wenqing Xu

    Article

    Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

    Insert

    TSC1 (939-992)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKD286

    Plasmid

    #125074

    Purpose

    Expression of SO_4622 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4622 (SO_4622 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKD276

    Plasmid

    #125069

    Purpose

    Expression of SO_0621 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_0621 (SO_0621 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKD250.2

    Plasmid

    #125077

    Purpose

    Expression of torR under PLtetO-1, output promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    torR (torR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR300.3

    Plasmid

    #125079

    Purpose

    Expression of uhpA under PLtetO-1, output Promoter PuhpT99

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    uhpA (uhpA )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107300

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge