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pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

Plasmid

#238184

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Synthetic, Human)

Use

Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-NeoR-LoxP

Plasmid

#227503

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPMS-2397

Plasmid

#231044

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to chicken Fc

Use

Affinity Reagent/ Antibody

Tags

8xHis

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-LynG

Plasmid

#206867

Purpose

Expression of LynG probe - LynAnchor-eGFP

Depositor

Dirk-Peter Herten

Article

Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

Insert

LynG

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Contains Lyn amino acids 1-16

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-Q61L

Plasmid

#214288

Purpose

Express constitutively active Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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2C-EGFP-t2a-mCD4

Plasmid

#216213

Purpose

Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

Depositor

Michelle Percharde

Article

Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

Insert

CD4 (Cd4 Mouse)

Tags

GFP-T2A

Expression

Mammalian

Mutation

CD4 transmembrane domain only

Promoter

MERVL

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTOCK1

Plasmid

#215566

Purpose

Escherichia coli–Aspergillus spp. shuttle cosmid-based library system

Depositor

Takuji Oka

Article

Kadooka et al J Fungi (Basel). 2024 Feb 29;10(3):188.

Inserts

AopyrG
AMA1
ampicillin resistance (bla) ORF

Use

Escherichia coli-aspergillus spp. shuttle cosmid …

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPS

Plasmid

#214901

Purpose

expression of the truncated variants of the SHIP2 without phosphatase domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 419-739 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 419-739aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA_OROV_NSm

Plasmid

#208935

Purpose

Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

Depositor

Gerald Barry

Insert

NSm

Tags

HA

Expression

Mammalian

Mutation

Deleted 949 bp from 5' end (Gn), deleted 29…

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il13

Plasmid

#207125

Purpose

Luciferase vector containing 3'UTR for mouse Il13

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-SUMO1

Plasmid

#114388

Purpose

pDONR vector with SUMO1 gene

Depositor

Vincent Dion

Article

Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

Insert

SUMO1 (SUMO1 Human)

Expression

Mammalian

Mutation

Q29H compared with NCBI reference NP_003343.1

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-ESRRG

Plasmid

#170684

Purpose

doxycycline-inducible overexpression of ESRRG

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ESRRG (ESRRG Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM + Ezrin

Plasmid

#202418

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

Ezrin and GFP

Mutation

delete PDZ binding motif

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A

Plasmid

#202732

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-INPP5ED477N

Plasmid

#202738

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

TagBFP2-FKBP-INPP5ED477N (INPP5E, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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