We narrowed to 22,137 results for: MUT

1. B-HypaSpCas9

   Plasmid

   #126763

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A; M694A; Q695A; H698A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AdMKP-3 S159AS197A

   Plasmid

   #131523

   Purpose

   Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Mutation

   changed Serine 159 to Alanine and Serine 197 to A…

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO-ORF66 C423A-2xStrep

   Plasmid

   #131111

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 423 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO-ORF66 C399A-2xStrep

   Plasmid

   #131112

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-ORF66 C393A-2xStrep

   Plasmid

   #131113

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 393 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/TO-ORF66 C355A-2xStrep

   Plasmid

   #131114

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 355 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-ORF66 C344A-2xStrep

   Plasmid

   #131115

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 344 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-ORF66 C341A-2xStrep

   Plasmid

   #131116

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 341 muta…

   Promoter

   CMV

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO-ORF66 C301A-2xStrep

    Plasmid

    #131117

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 301 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO-ORF66 C298A-2xStrep

    Plasmid

    #131118

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 298 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO-ORF66 C295A-2xStrep

    Plasmid

    #131119

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 295 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO-ORF66 C289A-2xStrep

    Plasmid

    #131120

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 289 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO-ORF66 C244A-2xStrep

    Plasmid

    #131121

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 244 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-ORF66 C427A-2xStrep

    Plasmid

    #131109

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 427 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-ORF66 C424A-2xStrep

    Plasmid

    #131110

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 424 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

    Plasmid

    #128383

    Purpose

    expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted

    Depositor

    Kalle Gehring

    Article

    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-

    Insert

    UbcH7 (UBE2L3 Human)
    

    Tags

    Bactrocera dorsalis parkin with mutation C463A an…

    Expression

    Bacterial

    Mutation

    mutation C86K in UbcH7 gene

    Promoter

    lac promotor

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PHAGE-P CMVt N-HA hE6-AP I (G20V)

    Plasmid

    #119308

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (G20V) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    G20V

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHAGE-P CMVt N-HA hE6-AP I (C21Y)

    Plasmid

    #119304

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (C21Y) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    C21Y

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

    Plasmid

    #119313

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Only AA 55-376

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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