We narrowed to 17,425 results for: mal.2

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pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-TPD54

Plasmid

#172454

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Tags

GFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p7129 pHAGE-P-CMVt-N-HA-GAW-HM13

Plasmid

#100155

Purpose

Expresses HM13

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

NM_030789.3 (HM13 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-Mart1-SABR

Plasmid

#119052

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

A0201-Mart1-SABR

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-SABR-Backbone

Plasmid

#119051

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFr.WPRE.SV40

Plasmid

#98929

Purpose

AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HA-EglN3-pcDNA3

Plasmid

#18960

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pAAVS1-shRNA

Plasmid

#82697

Purpose

For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

RNAi

Promoter

hPGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-AtAFB2-mCherry

Plasmid

#129718

Purpose

AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-SorLA-v2

Plasmid

#154900

Purpose

Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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901 pLNCX myr HA Akt1

Plasmid

#9005

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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1013 pcDNA3 T7 Akt1 T308A S473A

Plasmid

#9030

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 T308A S473A (AKT1 Human)

Expression

Mammalian

Mutation

T308A S473A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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MAC-CTSL

Plasmid

#172414

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Procathepsin L (Cathepsin L1) (CTSL Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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