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Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

Plasmid

#130665

Purpose

Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40

Use

Ae. aegypti expression

Expression

Insect

Promoter

Aedes aegypti poly-ubiquitin

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-ArchT-GFP

Plasmid

#86536

Purpose

Retrovirus expressing optogenetic silencer ArchT-GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

ArchT

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

Synapsin I (human)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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sh-Myocardin

Plasmid

#100768

Purpose

expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

sh-Myocardin

Use

RNAi

Promoter

H1

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-217

Plasmid

#26333

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-217 (Mir217 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-Zeo-PAX9

Plasmid

#75091

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85854

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-5p Sensor (MIR370 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-cdh5 TAL2

Plasmid

#41834

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

cadherin 5 (cdh5 Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91915

Purpose

Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cg2Cas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 V460P

Plasmid

#78814

Purpose

To overexpress RIPK3 V460P in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST_EWSR1_C2G_R650G

Plasmid

#26382

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

R650G

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1197

Plasmid

#26339

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1197 (Mir1197 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-216a

Plasmid

#26332

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-216a (Mir216a Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pRCIscript-crhr2_TAL1A

Plasmid

#44236

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1A

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1B

Plasmid

#44237

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1B

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-cdh5 TAL1

Plasmid

#41833

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

cadherin 5 (cdh5 Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-1964

Plasmid

#26343

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1964 (Mir1964 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-224

Plasmid

#26335

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-224 (Mir224 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only