We narrowed to 17,149 results for: tes
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Plasmid#136359PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
TagsTRX-HisExpressionBacterialPromoterT7Available SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PPR2
Plasmid#136360PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
TagsTRX-HisExpressionBacterialPromoterT7Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRS406 ATG9 stopGFP
Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
TagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108376PurposeFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-5xA
Plasmid#92010PurposeExpresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
TagsLambda-N-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsExpressionYeastAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
ExpressionYeastPromoterCYC1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cathepsin S S21C/C25A
Plasmid#84695Purposefor expression of mutant Cathepsin S in Leishmania tarantolaeDepositorInsertcathepsin S (CTSS Human)
UseExpression in leishmania tarantolaeTagshis-tagMutationS21C, C25APromoterunknownAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
625_HOXD13 in pmiRGlo
Plasmid#78129Purposeluciferase reporter for miRNA activityDepositorInsertHOXD13 (HOXD13 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag MFF iso1 S172A
Plasmid#74391PurposeFlag tagged human S172A MFF isoform 1 mutantDepositorInsertFlag-MFF (MFF Human)
TagsFlagExpressionMammalianMutationdelta exon 1 (1-26) and Ser 172 Ala.Available SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-ΔCT
Plasmid#64062Purposemyc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationDeletion of C-terminal Asp338–Ser443Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
ExpressionWormAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A(partial)
Plasmid#53705PurposeLuciferase reporter assay for microRNA binding on CIP2A coding sequenceDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41
Plasmid#52720Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 328–447Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-GST-Presc-mAkt1 (S473T)
Plasmid#48806Purposeexpresses mutant Akt1DepositorAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-FOXO3 R248K R250K
Plasmid#52427Purposemammalian expression of FOXO3 methylation resistant mutantDepositorInsertFOXO3a (FOXO3 Human)
TagsHAExpressionMammalianMutationR248K R250K, mutant methylation sitesAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT MEK5-DD-mRFP1
Plasmid#47547PurposeGateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutantDepositorAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW-EGFP-2xmiR-1 (pAW-AS-21)
Plasmid#40832PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-1
UseMirna reporterTagsEGFPExpressionInsectPromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only