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We narrowed to 18,351 results for: multi

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Showing: 9501 - 9520 of 18351 results

  1. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Publication
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Synthetic, Human)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186355-plasmid-map-sequence-id-373423

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  2. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  3. Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R]

    Plasmid
    #114507
    Purpose
    Mammalian Expression Plasmid of anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Human). Derived from hybridoma N120A/9.1.
    Depositor
    James Trimmer
    Publication
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Homo sapiens) recombinant mouse monoclonal antibody (NRG1 Mouse)
    Expression
    Mammalian
    Promoter
    dual CMV
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    114507-plasmid-map-sequence-id-245886

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  4. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Publication
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

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  6. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

    Plasmid
    #181695
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Publication
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181695-plasmid-map-sequence-id-354495

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  8. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Publication
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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  9. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  10. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Publication
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  11. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Publication
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  13. pBT259_(pRosa26-G-tTA2)

    Plasmid
    #36878
    Depositor
    Liqun Luo
    Publication
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP
    tTA2
    beta-globin intron
    Neo
    diphteria toxin A
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36878-plasmid-map-sequence-id-46122

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  15. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  16. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Publication
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    173158-plasmid-map-sequence-id-358594

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  17. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Publication
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

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  19. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid
    #246435
    Purpose
    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV
    Available since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246435-plasmid-map-sequence-id-488935

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  20. MB 45t3 thsS(t3)R-sfGFP_mCherry

    Plasmid
    #232470
    Purpose
    Optimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Publication
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232470-plasmid-map-sequence-id-467797

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Showing: 9501 - 9520 of 18351 results