We narrowed to 19,007 results for: rev
-
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-EGFP-RIP2
Plasmid#131201Purposemammalian expression, tet RDepositorAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_MARV
Plasmid#135472PurposeMammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoterDepositorInsertVP30
ExpressionMammalianMutationNonePromoterAGAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
TagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KAC1001(D1957A)
Plasmid#139312PurposeRecruitable PI4KA kinase-dead catalytic fragmentDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
ExpressionMammalianMutationD1957A, G1986DAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-OFF hM3Dq-mCherry
Plasmid#161578PurposeTo express hM3Dq in cells not expressing Cre.DepositorInserthM3Dq-mCherry
UseAAVMutationNAPromoterhSynAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.4.2
Plasmid#97364PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.2
Plasmid#97357PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-CKAR2
Plasmid#236097PurposeCytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertExRai-CKAR2-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαi*-BERKY3
Plasmid#158424PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsmycPromoterCMVAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMCtKq28-TSC10M1-sspB
Plasmid#49526PurposeKanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only