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pIT5_SL

Plasmid

#173769

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBT100.110

Plasmid

#122602

Purpose

clone CDA1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp3te

Plasmid

#116055

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp3te

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p4g3R

Plasmid

#107958

Purpose

RFP reporter [PT7(-3G)-RBSB0034, low copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-L

Plasmid

#222847

Purpose

Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Expression

Yeast

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCFT7.2

Plasmid

#208808

Purpose

Expresses the fluorescent protein FAST

Depositor

François-Xavier Campbell-Valois

Article

Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

Insert

FAST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pZD-CJ1111

Plasmid

#175058

Purpose

rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMAP L2B

Plasmid

#153357

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMAP L1Γ

Plasmid

#153345

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMAP L1B

Plasmid

#153344

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL724

Plasmid

#140394

Purpose

Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-hucO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL716

Plasmid

#140386

Purpose

Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-mphO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJKW0770

Plasmid

#119023

Purpose

E.coli expression plasmid for PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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B7-2

Plasmid

#111490

Purpose

Gln-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Gln-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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B2-1

Plasmid

#111468

Purpose

Ile-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ile-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-TEVPZ

Plasmid

#116065

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

TEVPZ

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-rapTEVPZ

Plasmid

#116076

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

rapTEVPZ

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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