We narrowed to 9,485 results for: Coli

1. pBN216

   Plasmid

   #167963

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBN217

   Plasmid

   #167964

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN261

   Plasmid

   #167966

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN262

   Plasmid

   #167967

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E, Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBRC901

   Plasmid

   #167948

   Purpose

   express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBN008

   Plasmid

   #167949

   Purpose

   express E. coli E69 (O9a:K30) Wzc with K540M mutation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN202

   Plasmid

   #167950

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN093

   Plasmid

   #167951

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, delete motif 3

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AlkB-D135G

   Plasmid

   #160434

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Glycine; deleted the…

   Promoter

   T7

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVR

    Plasmid

    #84718

    Purpose

    E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCML 314

    Plasmid

    #98584

    Purpose

    pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Inserts

    CsrA
    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMART-Ala1

    Plasmid

    #65814

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Insulated waaHp from E. coli

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLRD01

    Plasmid

    #208257

    Purpose

    Production of terpentetriene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cyc1
    cyc2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAT5

    Plasmid

    #112581

    Purpose

    Used for expression of protein in E. coli as non-fusions without initiation ATG

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBad-HPII

    Plasmid

    #105839

    Purpose

    expresses E. coli HPII catalase with N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLRD26

    Plasmid

    #208273

    Purpose

    Production of levopimaradiene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    LPS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHL 233

    Plasmid

    #37754

    Depositor

    Han Lim

    Article

    Hussein et al Nucleic Acids Res. 2012 May 22.

    Inserts

    ptet_ompC_gfp
    pLac_st7 TetR_mCherry
    

    Use

    Synthetic Biology; E. coli

    Tags

    gfp and mCherry

    Expression

    Mutation

    Promoter

    pLac and ptet

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLRD25

    Plasmid

    #208272

    Purpose

    Production of cis-abienol in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    abCAS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24a_thyA_R166Q_untagged

    Plasmid

    #191307

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R166Q TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a_thyA_Q33S_untagged

    Plasmid

    #191308

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q33S TYMS

    Promoter

    T7

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge