We narrowed to 8,384 results for: Dos

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pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

Plasmid

#183528

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet

Use

AAV

Mutation

I146M/G174S

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183529

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

Ef1a

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 blast

Plasmid

#104997

Purpose

This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFB2B

Plasmid

#195284

Purpose

Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Puro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mClover3

Plasmid

#105795

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mClover3

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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scAAV-Cre

Plasmid

#177933

Purpose

Expresses Cre-recombinase and can be packaged into AAV particles.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Insert

CAG-Cre

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL2

Plasmid

#23909

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL2 (ABL2 Human)

Use

Gateway Cloning

Mutation

K115M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MST1R

Plasmid

#23942

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MST1R (MST1R Human)

Use

Gateway Cloning

Mutation

R322Q

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKA

Plasmid

#23532

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKA (AURKA Human)

Use

Gateway Cloning

Mutation

I57V

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK4

Plasmid

#23713

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK4 (PAK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAR1A

Plasmid

#23741

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1A (PRKAR1A Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-Pericam

Plasmid

#87381

Purpose

Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

Mito-Pericam [ratiometric]

Use

Retroviral

Expression

Mammalian

Promoter

Retroviral 5' LTR promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-EIF2AK4

Plasmid

#23840

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EIF2AK4 (EIF2AK4 Human)

Use

Gateway Cloning

Mutation

F15S

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PTK2B

Plasmid

#23898

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2B (PTK2B Human)

Use

Gateway Cloning

Mutation

K42M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAB1

Plasmid

#23360

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB1 (PRKAB1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CAMK2G

Plasmid

#23409

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK2G (CAMK2G Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
scAAV-sgRNA-GFP

Plasmid

#177935

Purpose

Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-IRAK4

Plasmid

#23749

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IRAK4 (IRAK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only