We narrowed to 3,940 results for: fas

1. pDDGFP-Leu2d-GgKDELR2 R159A

   Plasmid

   #123625

   Purpose

   Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg159Ala

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104619

   Purpose

   Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR1316

   Plasmid

   #111126

   Purpose

   Expresses FANCA.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCA (FANCA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-EGFP-RhoA-wt

   Plasmid

   #12965

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. VPS13A^mCherry

   Plasmid

   #118758

   Purpose

   VPS13A internally tagged with mCherry at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   mCherry inserted at position 1372

   Expression

   Mammalian

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pre_IV_CS10_1545_v1

    Plasmid

    #126861

    Purpose

    4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS10_1545_v1
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pre_IV_CS10_1033_v1

    Plasmid

    #126860

    Purpose

    4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS10_1033_v1
    

    Use

    Synthetic Biology

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. QUIN

    Plasmid

    #184267

    Purpose

    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.

    Depositor

    Feng Qu

    Article

    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

    Insert

    CPSMV RNA1 cDNA with 4 introns
    

    Expression

    Plant

    Mutation

    S1098P, K1651R

    Promoter

    CaMV 35S with duplicated enhancer

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-EGFP-Rac1-wt

    Plasmid

    #12980

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pre_IV_CS8_7569_v1

    Plasmid

    #126865

    Purpose

    7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubation

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS8_7569_v1
    

    Use

    Synthetic Biology

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFS16 Pmyo-3 NPP-9 mChBioFLAG

    Plasmid

    #45099

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    npp-9 (npp-9 Nematode)
    unc-119 (unc-119 Nematode)
    

    Tags

    BLRP, FLAG, and mCherry

    Expression

    Worm

    Promoter

    myo-3 and unc-119

    Available Since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDHblast-flx-sORF-Katushka

    Plasmid

    #179457

    Purpose

    Lentiviral vector encoding a CRE inducible Katushka gene

    Depositor

    Ton Schumacher

    Article

    Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

    Insert

    loxP-shuffledORF-loxP-Katushka
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ub[9]-mRFP[1]

    Plasmid

    #20249

    Depositor

    Nico Dantuma

    Article

    Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

    Insert

    ubiquitin C (UBC Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    changed Stop codon 686 to Glycine

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L1E-1

    Plasmid

    #21193

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Retroviral

    Mutation

    Mek1 R4F fused to ER*, constitutive active

    Available Since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pDDGFP-Leu2d-GgKDELR2

    Plasmid

    #123618

    Purpose

    Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CC2

    Plasmid

    #21207

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Subclone vector

    Mutation

    Mek1 R4F fused to ER*, constitutive active

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only