We narrowed to 2,385 results for: fas

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pDDGFP-Leu2d-GgKDELR2 R47K

Plasmid

#123621

Purpose

Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Lys

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127A

Plasmid

#123622

Purpose

Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Ala

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2t

Plasmid

#231764

Purpose

Expression of mGold2t in mammalian cells

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Mammalian

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

Plasmid

#189752

Purpose

Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Publication

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Publication

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRU44

Plasmid

#167666

Purpose

ENTRY vector for gRNA4 cloning under pAtU3 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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CFP-GIV-CT FA

Plasmid

#69533

Purpose

Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

Depositor

Pradipta Ghosh

Publication

Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

Insert

GIV (CCDC88A Human)

Tags

CFP

Expression

Mammalian

Mutation

C terminal amino acids S1660-S1870 of human GIV w…

Promoter

CMV

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Publication

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Tags

Golgi and ER export signal

Expression

Mammalian

Promoter

CMV IE94

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ M2rtTA_CAGG TetON-Sox10 with GFP

Plasmid

#115241

Purpose

RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Publication

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Inserts

SOX10 (SOX10 Human)
eGFP

Tags

GFP (separate polypeptide)

Expression

Mammalian

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Publication

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJS169

Plasmid

#48882

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Publication

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Insert

lacI with ssrA tag

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDR121

Plasmid

#37148

Depositor

Dale Ramsden

Insert

XRCC6 (XRCC6 Human)

Tags

H6

Expression

Insect

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-mGold2t-Tubulin-C-18

Plasmid

#231779

Purpose

Visualization of tubulin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Tubulin-C-18

Plasmid

#231771

Purpose

Visualization of tubulin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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