We narrowed to 3,935 results for: gast

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pRSFDuet-1 IntS11 AA 300-597

Plasmid

#199329

Purpose

Expresses His-tagged Drosophila IntS11 amino acids 300-597

Depositor

Jeremy Wilusz

Publication

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 11 (IntS11 Fly)

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 300-597 only

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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p Pcrg1: Khd4-Ada-Gfp (CbxR)

Plasmid

#203734

Purpose

Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, fol

Depositor

Michael Feldbrügge

Publication

Sankaranarayanan et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

Khd4-Ada-Gfp

Tags

Gfp

Expression

Bacterial

Promoter

Pcrg1

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_B

Plasmid

#145912

Purpose

Insect Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Insect

Mutation

del 158-160 (GGG)

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1

Plasmid

#174493

Purpose

bacterial expression of Drosophila Sep1

Depositor

Manos Mavrakis

Publication

Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

Insert

Sep1 (Sep1 Fly)

Tags

His6-TEV

Expression

Bacterial

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1045C

Plasmid

#125005

Purpose

Expresses gRNAs targeting hid, eve, and wingless

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMCh-139

Plasmid

#92287

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

Insert

dGW182 NED (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 NED: aa 205-490

Promoter

Ac5

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Publication

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-Fc-His

Plasmid

#72190

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 Sense

Plasmid

#69892

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 1288–1457

Plasmid

#59055

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Bacterial

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST YFP Rab6 DN

Plasmid

#58448

Purpose

expression of drosophila YFP-Rab6 dominant negative mutant

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Tags

YFP

Expression

Insect

Mutation

T26N (dominant negative)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Marc Montminy, Biao Wang

Publication

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rholike WT

Plasmid

#52239

Purpose

Drosophila expression of YFP-Rholike

Depositor

Matthew Scott

Insert

Rholike (RhoL Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab18 CA

Plasmid

#37719

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

A64L (constitutive active)

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
slpr genomic fragment

Plasmid

#42074

Depositor

Norbert Perrimon

Publication

Stronach et al Genes Dev. 2002 Feb 1;16(3):377-87.

Insert

slpr genomic fragment (slpr Fly)

Expression

Insect

Promoter

Hsp70

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-92b

Plasmid

#22709

Depositor

Elisa Izaurralde

Publication

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro-RNA-92b (mir-92b Fly, primary transcript)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-9b

Plasmid

#22710

Depositor

Elisa Izaurralde

Publication

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 9b (mir-9b Fly, primary transcript)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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