We narrowed to 2,377 results for: pET28

Showing: 941 - 960 of 2377 results

Results per page:

Sort results by:

pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
csd2_121-308,S166A/pET28b(+)

Plasmid

#83301

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Serine 166 …

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd2_121-308,T151A/pET28b(+)

Plasmid

#83300

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Threonine 1…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd2_121-308,Y197F/pET28b(+)

Plasmid

#83299

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Tyrosine 19…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd2_121-308,E165A/pET28b(+)

Plasmid

#83298

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Glutamic ac…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd6_17-330/pET28b(+) N-tag

Plasmid

#71353

Purpose

csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
csd6_4-330/pET28b(+) N-tag

Plasmid

#71351

Purpose

csd6, construct:4-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330/pET28b(+) N-tag

Plasmid

#71352

Purpose

csd6, construct:13-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_E110A/pET28b(+) N-tag

Plasmid

#71354

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Glutamic acid 110 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y133A/pET28b(+) N-tag

Plasmid

#71356

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 133 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_C176A/pET28b(+) N-tag

Plasmid

#71360

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Cysteine 176 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H160A/pET28b(+) N-tag

Plasmid

#71359

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 160 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H155A/pET28b(+) N-tag

Plasmid

#71357

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 155 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only