We narrowed to 6,901 results for: plasmid dna

1. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

   Plasmid

   #208174

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
   

   Use

   Tags

   Strep-tag

   Expression

   Mammalian

   Mutation

   Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Antares2 c-myc

   Plasmid

   #100027

   Purpose

   A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   Antares2
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3-NL63-D14

   Plasmid

   #172666

   Purpose

   encodes NL63 spike protein, for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
   

   Use

   Lentiviral and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   c-terminal deletion of 14aa

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HA

   Plasmid

   #235678

   Purpose

   Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna3.1_mouseST6galI

   Plasmid

   #178435

   Purpose

   murine St6gal1 expression plasmid

   Depositor

   Deborah Fass

   Article

   Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

   Insert

   St6gal1 (St6gal1 Mouse)
   

   Use

   Tags

   6xHIS-TEV Cleavage Site

   Expression

   Mammalian

   Mutation

   Coding sequnce for amino acids 85-403

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Flag Pu.1

   Plasmid

   #66974

   Purpose

   Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3_NSlmb-vhhGFP4

    Plasmid

    #35579

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)_mtZFN(-)2G

    Plasmid

    #62799

    Purpose

    mtZFN2G architecture bearing (-) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(-)2G
    

    Use

    Tags

    Internal FLAG tag immediately after F1B mitochond…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(-)_mtZFN(+)2G

    Plasmid

    #62798

    Purpose

    mtZFN2G architecture bearing (+) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(+)2G
    

    Use

    Tags

    Internal HA tag immediately after F1B mitochondri…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1-spikeRBD::∆ACE2 SURF

    Plasmid

    #206957

    Purpose

    Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

    Depositor

    Xiaokun Shu

    Article

    Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

    Inserts

    sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
    ACE2 (ACE2 Human)
    

    Use

    Tags

    cSURF, mCherry, and nSURF

    Expression

    Mammalian

    Mutation

    17-615 and RBD (319-541)

    Promoter

    CMV

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

    Plasmid

    #176060

    Purpose

    Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K164A and H165A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

    Plasmid

    #218461

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273AGlu substitution

    Promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

    Plasmid

    #218462

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273Asp substitution

    Promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-hrDNA-12.0

    Plasmid

    #206320

    Purpose

    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

    Depositor

    Brian McStay

    Article

    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

    Insert

    Intergenic spacer of human rDNA repeat
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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