We narrowed to 6,025 results for: plasmid dna

1. pcDNA3_NSlmb-vhhGFP4

   Plasmid

   #35579

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSlmb-vhhGFP4 (slmb Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CCR5

   Plasmid

   #98943

   Purpose

   Mammalian expression plasmid for untagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(-)_mtZFN(-)2G

   Plasmid

   #62799

   Purpose

   mtZFN2G architecture bearing (-) FokI domain

   Depositor

   Michal Minczuk

   Article

   Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

   Insert

   mtZFN(-)2G
   

   Use

   Tags

   Internal FLAG tag immediately after F1B mitochond…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)_mtZFN(+)2G

   Plasmid

   #62798

   Purpose

   mtZFN2G architecture bearing (+) FokI domain

   Depositor

   Michal Minczuk

   Article

   Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

   Insert

   mtZFN(+)2G
   

   Use

   Tags

   Internal HA tag immediately after F1B mitochondri…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-spikeRBD::∆ACE2 SURF

   Plasmid

   #206957

   Purpose

   Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

   Depositor

   Xiaokun Shu

   Article

   Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

   Inserts

   sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
   ACE2 (ACE2 Human)
   

   Use

   Tags

   cSURF, mCherry, and nSURF

   Expression

   Mammalian

   Mutation

   17-615 and RBD (319-541)

   Promoter

   CMV

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

   Plasmid

   #176059

   Purpose

   Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

   Plasmid

   #218461

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr1273AGlu substitution

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

   Plasmid

   #218462

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr1273Asp substitution

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-hrDNA-12.0

    Plasmid

    #206320

    Purpose

    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

    Depositor

    Brian McStay

    Article

    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

    Insert

    Intergenic spacer of human rDNA repeat
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag Myb

    Plasmid

    #66980

    Purpose

    Expression plasmid for mouse Myb; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Myb (Myb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-CTE-Luc

    Plasmid

    #60881

    Purpose

    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    Luc-CTE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) EPHB4 Antisense

    Plasmid

    #60640

    Purpose

    Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+) HIPK3 Antisense

    Plasmid

    #60635

    Purpose

    Negative Control for the pcDNA3.1(+) HIPK3 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Homeodomain-interacting protein kinase 3 (HIPK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-H2-18 Light chain

    Plasmid

    #216308

    Purpose

    Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3-SARS2-B.1.617.1

    Plasmid

    #172319

    Purpose

    expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.1 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

    Promoter

    CMV

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-hHSF1 WT

    Plasmid

    #71724

    Purpose

    plasmid expressing myc-his tag hHSF1 WT

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    MYC 6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-cmyc

    Plasmid

    #16011

    Purpose

    mammalian expression of human cmyc

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    cmyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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