Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,791 results for: plasmid for e coli

Clear keywords
Showing: 941 - 960 of 1791 results

  1. pTCF79 H-2D(b) (CD8 mut)

    Plasmid
    #180453
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b) (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, replacement of transmembrane domain with e…
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    180453-plasmid-map-sequence-id-409097

    Enlarge

  2. pE-SUMO CRYBA3

    Plasmid
    #80747
    Purpose
    Expression of human beta A3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA3 (CRYBA1 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80747-plasmid-map-sequence-id-153503

    Enlarge

  4. pE-SUMO CRYBA1

    Plasmid
    #80745
    Purpose
    Expression of human beta A1-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA1 (CRYBA1 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80745-plasmid-map-sequence-id-153525

    Enlarge

  5. Sc hk/P-RFA3-pCDFDuet

    Plasmid
    #240743
    Purpose
    Over-express his(10)-PKA kinase motif-PreScission protease site-Sc RFA3 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    hk/P-RFA3 (RFA3 Budding Yeast)
    Tags
    His(10) followed by PKA kinase motif follwed by P…
    Expression
    Bacterial
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240743-plasmid-map-sequence-id-482370

    Enlarge

  6. pET28a_HCK

    Plasmid
    #224239
    Purpose
    Expression of human HCK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224239-plasmid-map-sequence-id-459258

    Enlarge

  7. PAS_HeSAHS4

    Plasmid
    #215648
    Purpose
    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215648-plasmid-map-sequence-id-426093

    Enlarge

  8. PAS_RvSAHS4

    Plasmid
    #215646
    Purpose
    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215646-plasmid-map-sequence-id-426091

    Enlarge

  9. pJA12 Mamu-A1*001:01

    Plasmid
    #180442
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*001:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180442-plasmid-map-sequence-id-355627

    Enlarge

  10. pJA13 Mamu-A1*002:01

    Plasmid
    #180443
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*002:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180443-plasmid-map-sequence-id-355622

    Enlarge

  11. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-B*008:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180455-plasmid-map-sequence-id-355081

    Enlarge

  13. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Promoter
    Trc
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

    Enlarge

  15. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Promoter
    lac
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53286-plasmid-map-sequence-id-83099

    Enlarge

  18. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

    Enlarge

  19. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

    Enlarge

  20. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

    Enlarge

Showing: 941 - 960 of 1791 results