We narrowed to 1,196 results for: post

1. pEGFPC2-gephyrin S268A/270A

   Plasmid

   #199608

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268A/S270A

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCPF101-pfQC-WT

   Plasmid

   #184877

   Purpose

   expresses soluble plasmodium falciparum QC protein with a histag

   Depositor

   Ferenc Scheeren

   Article

   Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   pfQC-wt
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR3-FLAG-Gephyrin E

   Plasmid

   #68819

   Purpose

   Flag tagged mammalian expression of E domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   internal EcoRI site has silent mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. MBP-rPKL WT

   Plasmid

   #107156

   Purpose

   MBP-rPKL WT for production and purification of rat PKL WT

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Use

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Mm_MyoGEF(WT)

   Plasmid

   #136325

   Purpose

   Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

   Depositor

   Marcos Malumbres, Guillermo de Cárcer

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hETFDH CRISPR_1

   Plasmid

   #232309

   Purpose

   Human ETFDH Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mEtfdh CRISPR_2

   Plasmid

   #232313

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEtfdh CRISPR_1

   Plasmid

   #232312

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hETFDH CRISPR_2

   Plasmid

   #232310

   Purpose

   Human ETFDH Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFPC2-gephyrin S268E/270E

    Plasmid

    #199609

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268E/S270E

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

    Plasmid

    #185717

    Purpose

    AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ala 11 to Pro, Val 70 to Pro

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PWPXLD-EGFR-P667A

    Plasmid

    #133750

    Purpose

    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Mutant P667A EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    proline 667 converted to alanine (Please see depo…

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-Mm_MyoGEF(T575A)

    Plasmid

    #136328

    Purpose

    Mouse MyoGEF-T575A mutant in Gateway pENTR

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    T575A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmirGlo-CALB2-3'UTR delta ARE&mir30

    Plasmid

    #74430

    Purpose

    Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid

    #196196

    Purpose

    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    

    Use

    Homology repair donor

    Tags

    V5-APEX2-mScarlet-I

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO/GFP-GIGYF1

    Plasmid

    #141188

    Purpose

    Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    GIGYF1 (GIGYF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-hPKL WT

    Plasmid

    #107159

    Purpose

    For mammalian expression of Flag-hPKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    Flag-hPKL WT (PKLR Human)
    

    Use

    Retroviral

    Tags

    N-term Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. retro-gfp-puro vector

    Plasmid

    #58249

    Purpose

    Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rat Alpha2 delta-1 pMT2

    Plasmid

    #58726

    Purpose

    expression of rat alpha2 delta-1 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge