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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,055 results for: crispr grnas

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Showing: 9661 - 9680 of 14055 results

  1. pYPQAT34

    Plasmid
    #223406
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223406-plasmid-map-sequence-id-454165

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  2. pYPQAT35

    Plasmid
    #223407
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223407-plasmid-map-sequence-id-452579

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  3. pYPQAT36

    Plasmid
    #223408
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223408-plasmid-map-sequence-id-454249

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  5. pTJK475

    Plasmid
    #138014
    Purpose
    SIX1 CRISPRi
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA13 (SIX1 Human)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138014-plasmid-map-sequence-id-275065

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  6. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  7. pMOD_C2515

    Plasmid
    #91083
    Purpose
    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    AtU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91083-plasmid-map-sequence-id-182047

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  14. pMOD_C2518

    Plasmid
    #91087
    Purpose
    Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    TaU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91087-plasmid-map-sequence-id-182052

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  15. pMOD_C2616

    Plasmid
    #91090
    Purpose
    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91090-plasmid-map-sequence-id-182055

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  16. pMOD_C2517

    Plasmid
    #91086
    Purpose
    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    TaU3
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91086-plasmid-map-sequence-id-182051

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  17. pMOD_C2520

    Plasmid
    #91089
    Purpose
    Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    OsU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91089-plasmid-map-sequence-id-182054

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  18. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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  19. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

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Showing: 9661 - 9680 of 14055 results