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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-hAgo2

Plasmid

#11590

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

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p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMC-6-ZmBbm-P2A-ZmWus2-9

Plasmid

#197750

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmBbm-P2A-ZmWus2 bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CD4-sTb(C)-M13-GFP

Plasmid

#219784

Purpose

sTb(C) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSIBR004

Plasmid

#177667

Purpose

SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pANCH2-Nat

Plasmid

#87249

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Expression

Yeast

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (115 - 575)

Plasmid

#110750

Purpose

Bacterial expression of PhPINK1 (biochemistry)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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JD136

Plasmid

#229497

Purpose

Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, L4-22k/33k, VA RNA I

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-UFM1

Plasmid

#134640

Purpose

Expression of FLAG-tagged human UFM1 wildtype

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2high-mCherry

Plasmid

#104063

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBTR1

Plasmid

#22468

Depositor

Gary Pielak

Article

Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

Insert

cytochrome c (CYCS Human)

Expression

Bacterial

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pMC-6-Ruby-9

Plasmid

#197740

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ruby reporter tricistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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