We narrowed to 14,905 results for: sta
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Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
TagsHALO-tag, 6xHISExpressionBacterialPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 508)
Plasmid#195030PurposeGAL4-Creb5 fusion construct (full length, 508aa)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
ExpressionMammalianAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-COX2-EBFP2
Plasmid#184720PurposeExogenous COX2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCOX2-EBFP2 (PTGS2 Synthetic, Human)
ExpressionMammalianAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHA_SBV_NSs
Plasmid#208932PurposeExpresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNSs
TagsHAExpressionMammalianMutationDeleted 50 bp from 5' end (including start c…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB1-B-TelR
Plasmid#204980PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D1-TelR
Plasmid#204983PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB3-B-TelR
Plasmid#204992PurposeLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-Myr-PTEN
Plasmid#206983PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signalDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsN-terminal myristoylation siteExpressionMammalianPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-GFP
Plasmid#199222PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrtTA-Advanced::P2A::EGFP
TagsEGFPExpressionMammalianPromotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K113R-3xFlag_IRES-GFP
Plasmid#198391PurposeRPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site mutant (K113R)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CFL
Plasmid#131457PurposeEncodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionBacterial and MammalianMutationR15C (in DYSF) and Q2579H (in CFL)Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyMutationAll BsaI, Esp3I and BpiI restriction sites were r…Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-1
Plasmid#110366PurposeMammalian expression of HisA-tagged HOXC6-1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHOXC6 (HOXC6 Human)
TagsHISExpressionMammalianMutationlong isoformAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD96_hIRG1_4-461_pvp008
Plasmid#124874PurposeExpresses hCAD Asn152Ser mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated Asn152 to Ser, deleted amino acids 1-3 an…PromoterT7Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG3-srt-his
Plasmid#124805PurposeHDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMurine IgG3
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only