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CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHCT2

Plasmid

#130970

Purpose

HyPr plasmid with natMX marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Expression

Yeast

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHRW32

Plasmid

#130972

Purpose

HyPr plasmid with ble marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Expression

Yeast

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDMS197

Plasmid

#43831

Depositor

Dieter Schifferli

Article

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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pAID5.3-C

Plasmid

#145813

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCM66

Plasmid

#45827

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Broad host range cloning vector in bacteria

Promoter

lac

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p359_DG

Plasmid

#234702

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p356_DG

Plasmid

#234699

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAID5.1-N

Plasmid

#145808

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-THRX-TEV-GG

Plasmid

#215796

Purpose

Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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human Alpha-synuclein

Plasmid

#51437

Purpose

human codon optimized Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (SNCA Human)

Use

Cloning

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRB895

Plasmid

#124586

Purpose

C-terminal fusions of C. albicans optimized mNeonGreen fluorescent protein.

Depositor

Richard Bennett

Article

Frazer et al Curr Protoc Microbiol. 2019 Feb 12:e76.

Type

Empty backbone

Use

Synthetic Biology; Candida albicans expression

Tags

mNeonGreen

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUCX

Plasmid

#60681

Purpose

E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression

Depositor

David Ackerley

Article

Prosser et al Biotechnol Lett. 2014 Sep 26.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAID5.3-N

Plasmid

#145812

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-ccdB

Plasmid

#14147

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pRSCq3

Plasmid

#236106

Purpose

Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning vector carrying ampicillin resistance gene.

Depositor

Dehong Zheng

Article

Huang et al Phytopathol Res (2025) 7, 78. https://do

Type

Empty backbone

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRE107

Plasmid

#43829

Depositor

Dieter Schifferli

Article

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAID5.4-N

Plasmid

#145814

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID-EGFP

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.4-C

Plasmid

#145815

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID-EGFP

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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