We narrowed to 24,868 results for: ina

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FRB-CFP-ras

Plasmid

#87451

Purpose

rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

FRB (MTOR Human)

Tags

CFP and ras fragment

Expression

Mammalian

Mutation

FKBP and rapamycin binding domain only

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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NEK1_HUMAN_D0

Plasmid

#79725

Purpose

This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK1 (NEK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB121

Plasmid

#68575

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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Flrt1-Fc-His

Plasmid

#72073

Purpose

Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt1 (Flrt1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (OTU, aa 133-293)

Plasmid

#61419

Purpose

Expresses human OTUD6B (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-132.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTXG-hTDG-K330A

Plasmid

#52282

Purpose

Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Mutation

K330A, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pHJ41

Plasmid

#48360

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pWPT-/CGA-mEGFP-IRES-mCherry

Plasmid

#49233

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-ILKAP

Plasmid

#34817

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab10 DN

Plasmid

#37709

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T23N (dominant negative)

Promoter

GAL4

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCY 3010-02

Plasmid

#36210

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yeast-enhanced (yE) polylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-hphMX4

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-M2-PAK2 S20A

Plasmid

#31664

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

Changed Serine 20 to Alanine

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Barkor (∂CC)

Plasmid

#24404

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 91-180 of the coiled-coil…

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaST1

Plasmid

#22632

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST1 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST1 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaST2

Plasmid

#22633

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST2 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST2 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only