We narrowed to 15,899 results for: grna

1. p426*-SaSgH

   Plasmid

   #136994

   Purpose

   Helper plasmid for SaCas9 gRNA cloning and expression

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GluA2 (px330:2-guideCas9)

   Plasmid

   #169420

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP2832

   Plasmid

   #160672

   Purpose

   Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   gmc6 (gfp) sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP2820

   Plasmid

   #160669

   Purpose

   Mobile CRISPRi sfGFP 'test', empty sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   empty sgRNA (BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TtRMPVIR

   Plasmid

   #27995

   Depositor

   Scott Lowe

   Article

   Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

   Insert

   TtRMPVIR (shRen)
   

   Use

   RNAi and Retroviral; Fluorescent reporters

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPGUw-HACK-G4>QF2

   Plasmid

   #80277

   Purpose

   GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG244 iFAST

    Plasmid

    #130809

    Purpose

    Expresses iFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GluN1 (px330:2-guideCas9)

    Plasmid

    #169426

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV.MRE11i

    Plasmid

    #15565

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

    Insert

    MRE11 shRNA (MRE11 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

15. GluN2A (px330:2-guideCas9)

    Plasmid

    #169428

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GluN2B (px330:2-guideCas9)

    Plasmid

    #169430

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS-shTGFbeta1

    Plasmid

    #14973

    Depositor

    Joan Massague

    Article

    Thomas et al Cancer Cell. 2005 Nov . 8(5):369-80.

    Insert

    TGFbeta1 shRNA (Tgfb1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

19. GEPIR Sox4.2137

    Plasmid

    #101119

    Purpose

    shRNA Vector

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    Sox4.2137
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GEPIR Sox4.2091

    Plasmid

    #101118

    Purpose

    shRNA vec

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    Sox4.2137
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only