We narrowed to 16,466 results for: pho;

1. pRK5-PLBD2-6xHis S392A

   Plasmid

   #213607

   Purpose

   Expresses PLBD2-6xHis S392A in mammalian cells

   Depositor

   Monther Abu-Remaileh

   Article

   Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

   Insert

   phospholipase B domain containing 2 (PLBD2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Changed serine 392 to alanine

   Promoter

   CMV

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b-ITPA

   Plasmid

   #60824

   Purpose

   to purify human ITPA from bacteria

   Depositor

   Youri Pavlov

   Article

   Porta et al Acta Crystallogr Sect F Struct Biol Crys

   Insert

   ITPA (ITPA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

   Plasmid

   #223721

   Purpose

   Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted sterile alpha motif domain (deleted amino…

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRB133.2

   Plasmid

   #181950

   Purpose

   aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial

   Mutation

   Promoter

   PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Stat3 S727A pRc/CMV

   Plasmid

   #8708

   Depositor

   Jim Darnell

   Article

   Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

   Insert

   Stat3 S727A (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S727A (exhibits o serine phosphorylation either c…

   Promoter

   Available Since

   Aug. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-HSF1Δ∼PRD

   Plasmid

   #71725

   Purpose

   plasmid expressing mic-his tag phosphorylation-deficient hHSF1

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   phosphorylation-deficient HSF1 (HSF1 Human)
   

   Use

   Tags

   mac 6xHIS

   Expression

   Mammalian

   Mutation

   15 phosphorylation sites in RD mutated to Alanine

   Promoter

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCapV

   Plasmid

   #183028

   Purpose

   the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase CapV
   

   Use

   Tags

   No

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNAS1b split mCherry NEDD4 WW2 neg ctrl

   Plasmid

   #112030

   Purpose

   Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
   

   Use

   Tags

   Split mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

   Plasmid

   #107163

   Purpose

   Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

   Depositor

   Jose Carlos Cameselle

   Article

   Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

   Insert

   F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid

    #112029

    Purpose

    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    

    Use

    Tags

    Split mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge